Journal: Experimental and Therapeutic Medicine

Article Title: Scutellaria barbata D. Don inhibits growth and induces apoptosis by suppressing IL-6-inducible STAT3 pathway activation in human colorectal cancer cells

doi: 10.3892/etm.2015.2692

Figure Lengend Snippet: Effect of EESB on the HT-29 cell cycle. Cells were pretreated with various concentration of EESB for 1 h, followed by stimulation with 10 ng/ml IL-6 for 24 h. (A) Cells were stained with propidium iodide and analyzed by fluorescence-activated cell sorting. The proportion of DNA in the S phase was calculated using ModFit LT version 3.0 software. (B) Quantification of fluorescence-activated cell sorting analysis. The data shown are averages with standard deviation from 3 independent experiments. #P<0.05 vs. cells treated with IL-6 but not EESB. EESB, ethanol extract of Scutellaria barbata D. Don; IL-6, interleukin-6.

Article Snippet: The HT-29 cell cycle progression was determined through flow cytometric analysis using a propidium iodide (PI) staining cell cycle assay kit (BD Biosciences, Franklin Lakes, NJ, USA).

Techniques: Concentration Assay, Staining, Fluorescence, FACS, Software, Standard Deviation

Journal: Experimental and Therapeutic Medicine

Article Title: Scutellaria barbata D. Don inhibits growth and induces apoptosis by suppressing IL-6-inducible STAT3 pathway activation in human colorectal cancer cells

doi: 10.3892/etm.2015.2692

Figure Lengend Snippet: Effect of EESB on HT-29 cell apoptosis. Cells were pretreated with various concentration of EESB for 1 h, followed by stimulation with 10 ng/ml IL-6 for 24 h. (A) Cells were collected and stained with Annexin V/PI, followed by fluorescence-activated cell sorting analysis. Double-negative stained cells indicate the live cell population; Annexin V-positive/PI-negative stained cells and Annexin V/PI double-positive stained cells represent early and late apoptosis, respectively; Annexin V-negative and PI-positive stained cells show dead cells. (B) Quantification of fluorescence-activated cell sorting analysis. The data shown are averages with standard deviation from 3 independent experiments. #P<0.05 vs. cells treated with IL-6 but not EESB. EESB, ethanol extract of Scutellaria barbata D. Don; IL-6, interleukin-6; UL, upper left; UR, upper right; LR, lower right; LL, lower left; PI, propidium iodide; FITC, fluorescein isothiocyanate.

Article Snippet: The HT-29 cell cycle progression was determined through flow cytometric analysis using a propidium iodide (PI) staining cell cycle assay kit (BD Biosciences, Franklin Lakes, NJ, USA).

Techniques: Concentration Assay, Staining, Fluorescence, FACS, Standard Deviation

Journal: British journal of haematology

Article Title: Targeted inhibition of the deubiquitinating enzymes, USP14 and UCHL5, induces proteotoxic stress and apoptosis in Waldenström macroglobulinaemia tumour cells

doi: 10.1111/bjh.13304

Figure Lengend Snippet: b-AP15 induces tumour-specific apoptosis in WM cell lines and primary patient-derived WM cells. (A) All available WM cell lines (n = 6, WT and BR derivatives) were treated with b-AP15 at indicated concentrations and stained with annexin-V and propidium iodide followed by flow cytometry to examine apoptosis. Annexin-V positivity (apoptosis) was significantly observed in b-AP15-treated WM cells by 12 h in a dose-dependent manner (**P < 0·005). Each experiment was conducted a minimum of three times with control cells (no drug treatment) showing a viability (annexin-V positive and propidium iodide-negative population) of ≥85%. Following treatment, the percentage of cells affected by b-AP15 was calculated by normalizing data from treated cells relative to the control (untreated) cells. (B) Malignant CD19+/CD138+ WM cells from human patients (WM1 and WM2) and peripheral blood mononuclear cells (PBMCs, n = 2) were similarly stained with annexin-V and propidium iodide to assess apoptosis. Robust apoptotic cell death was noted in patient-derived WM cells after 12 h exposure to b-AP15 0·5 μmol/l. Contrastingly, minimal apoptosis (~13%) was noted in b-AP15-treated PBMCs exposed to the deubiquitinase enzyme inhibitor for 48 h. (C and D) Immunoblotting for PARP1 cleavage confirmed execution of apoptosis in both WM tumour cell lines and primary WM tumour cells. BR, bortezomib-resistant.

Article Snippet: Annexin-V and propidium iodide apoptosis staining kit was purchased from BD Biosciences (San Jose, CA, USA).

Techniques: Derivative Assay, Staining, Flow Cytometry, Western Blot

Journal: Heliyon

Article Title: Rapid Inactivation of mixed biofilms of Candida albicans and Candida tropicalis using antibacterial photodynamic therapy: Based on PAD™ Plus

doi: 10.1016/j.heliyon.2023.e15396

Figure Lengend Snippet: Photoactivated disinfection treatment. The mixed biofilms cultured for 24 h were incubated with 1 mg/mL toluidine blue O (TBO) for 1 min (A,D,G,J), 5 min (B,E,H,K), or 10 min (C,F,I,L), followed by exposure to LED for 1 min (A,B,C,G,H,I) or 2 min (D,E,F,J,K,L) with output power of 500 mW (A–F) or 750 mW (G–L). Output power/illumination time was arranged in each row, and incubation time was arranged in each column.Untreated biofilms were used as a negative control (M). SYTO9/propidium iodide staining was performed to identify live/dead fungi. Fungi were observed using a confocal laser scanning microscope. Green (A–M) fluorescence indicated live fungi and red (A,B,C,M) fluorescence indicated dead fungi. Orange (D,F,G,I,J) or Yellow (E,H,K,L) fluorescence is an overlay of green and red, indicating dead fungi. When Image J software was used to count the live/dead fungi on the image, the green channel and the red channel were counted respectively. Scale bar = 50 μm. The experiment was performed three times with three biological replicates. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: The live/dead fungi were detected by SYTO9 (green)/propidium iodide (PI; red) staining using a kit from Kehua Jingwei Technology (Beijing, China) following the manufacturer’s instruction.

Techniques: Cell Culture, Incubation, Negative Control, Staining, Laser-Scanning Microscopy, Fluorescence, Software